RNA Timestamps
Timestamps enables researchers to track not just which genes are present in a cell, but when those genes were expressed. In turn, this allows us to study how cascades of gene expression in response to specific stimuli can drive the body’s response to disease.
Category
Contextual Transcriptomics
People
Aaron Wagen
James Bayne
Ali Ghareeb
Collaborators
Description
We have engineered an RNA editing enzyme, ADAR, to attach a temporal barcode to each RNA, allowing the age of the RNA to be inferred by RNA sequencing. By measuring the ages of populations of RNA collected from a single cell, it is possible to infer the multiplicity, timing, duration, and magnitude of past transcriptional events.
Milestones
2017: Project launched
2020: Paper Published
2021: Project selected for funding by an Impetus Award