RNA Timestamps

Timestamps enables researchers to track not just which genes are present in a cell, but when those genes were expressed. In turn, this allows us to study how cascades of gene expression in response to specific stimuli can drive the body’s response to disease.

Category

Contextual Transcriptomics

People

Aaron Wagen

James Bayne

James Bayne

Ali Ghareeb

Ali Ghareeb

Description

We have engineered an RNA editing enzyme, ADAR, to attach a temporal barcode to each RNA, allowing the age of the RNA to be inferred by RNA sequencing. By measuring the ages of populations of RNA collected from a single cell, it is possible to infer the multiplicity, timing, duration, and magnitude of past transcriptional events.

Milestones

2017: Project launched
2020: Paper Published
2021: Project selected for funding by an Impetus Award

Paper

RNA Timestamps identify the age of single molecules in RNA sequencing 
Rodriques*, Chen* et al., Nature Biotechnology (2020)

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