Slide-seq
Slide-seq blends the concepts of microscopy with the concepts of single-cell RNA sequencing to provide rich genome-wide gene expression data with high spatial resolution. Slide-seq provides researchers with a new way to study the spatial organization of tissue, allowing them to visualize how different cell types are distributed throughout the tissue and how tissue organization is disrupted by disease.
Description
In Slide-seq, freshly frozen tissue can be sliced onto prepared arrays of DNA-barcoded beads, causing RNA in the tissue to transfer onto the beads. Subsequent library preparation yields data that is equivalent to single cell RNA sequencing data, but with a spatial location associated with each bead.
Milestones
2017: Project launched
2019: Paper Published